Fig. 1

Alignment analysis of reference genomes and novel transcript annotations. A Random distribution of Reads on the reference gene. The abscissa is the relative position of the gene, and the ordinate is the proportion of reads in the total alignment in the corresponding position interval, and different colors represent different samples. This reflects the coverage of the gene (along 5’ to 3’) by the reads. B Quantity distribution of different types of new transcripts. o is the other part of the same strand overlapping with the reference exon; j is at least one matching multi-exon; x is the exon overlap on the anti-strand; i is the intron completely contained in the reference transcript Middle; u is an unknown new transcript. C CDS length distribution of new transcripts. The abscissa indicates the length of reads; the ordinate indicates the number of reads within the length range, and the red dotted line indicates the length of N50. D Distribution of the number of new transcripts annotated by different databases