Table 4 Short-list of plasticity-responsive candidate genes based on their functional involvement in drought tolerance mechanisms

Traits

Chr.

Gene ID

Gene size

Gene annotation

Molecular function

Biological function

pTRL

1 A

TraesCS1A02G295000

1,549

protein self-association-unfolded protein binding (GO:0006950), abscisic acid binding-signaling receptor activity (GO:0009725),

response to heat- response to reactive oxygen species-response to salt stress (GO:0006950), abscisic acid-activated signaling pathway (GO:0009725)

TraesCS1A02G295400

629

water channel activity (GO:0009414), hydrolase activity(GO:0005886)

response to water deprivation (GO:0009414), carbohydrate metabolic process(GO:0005886)

TraesCS1A02G296200

4,331

structural molecule activity (GO:0006888)

response to freezing (GO:0050826), intracellular protein transport-vesicle-mediated transport (GO:0006888)

TraesCS1A02G296300

5,040

potassium ion leak channel activity(GO:0016021), actin filament binding-ATP binding(GO:0048765)

root hair elongation-vesicle transport along actin filament- root hair cell differentiation (GO:0048765) , lateral root development (GO:0048527)

1B

TraesCS1B02G269100

1,321

protein self-association-unfolded protein binding (GO:0006979), growth factor activity-growth hormone receptor binding-hormone activity(GO:0060416)

response to heat -response to reactive oxygen species -response to salt stress (GO:0006979), lateral root development (GO:0048527), positive regulation of growth-response to growth hormone (GO:0060416)

TraesCS1B02G272100

3,720

water channel activity- (GO:0006833), cellular response to water deprivation (GO:0042631), hydrolase activity (GO:0048046)

Transport-water transport (GO:0006833), apoplast (GO:0048046)

TraesCS1B02G272900

4,291

auxin-activated signaling pathway-transmembrane transport (GO:0009926)

Auxin signaling pathway, auxin polar transport (GO:0009926)

TraesCS1B02G269400

3,006

protein self-association-unfolded protein binding(GO:0009651), hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0005886)

response to heat-response to reactive oxygen species (GO:0009651), response to cold (GO:0009409)

TraesCS1B02G272000

2,745

water channel activity(GO:0009414)

response to water deprivation(GO:0009414),  response to cold (GO:0009409)

2 A

TraesCS2A02G144900

1,511

heme binding-metal ion binding-peroxidase activity(GO:0009505), chlorophyll binding (GO:0009628)

response to environmental stresses(GO:0009505), response to abiotic stimulus (GO:0009628)

TraesCS2A02G145000

1,181

chlorophyll binding (GO:0009628)

light harvesting in photosystem I-response to light stimulus (GO:0009628) peroxidase activity-environmental stress (GO:0004601)

2B

TraesCS2B02G409800

1,306

actin-dependent ATPase activity-actin filament binding-ATP binding- (GO:0048765), voltage-gated ion channel activity (GO:0009913)

actin filament organization-root hair elongation-vesicle transport along actin filament-root hair cell differentiation (GO:0048765)

TraesCS2B02G410400

4,291

protein self-association-unfolded protein binding  (GO:0006950)

response to heat- response to reactive oxygen species-response to salt stress - response to stress  (GO:0006950)

4B

TraesCS4B02G259600

3,639

ion channel binding(GO:1,903,959), protein-macromolecule adaptor activity(GO:0040008)

regulation of anion transmembrane transport (GO:1,903,959),response to starvation-positive regulation of cell growth (GO:0040008)

5 A

TraesCS5A02G551700

939

ATPase activity- ATP binding -unfolded protein binding(GO:0034605), DNA-binding transcription factor activity-sequence-specific DNA binding(GO:0009751)

cellular response to heat (GO:0034605), hyperosmotic salinity, and hormone response(GO:0009751)

pRAD

7 A

TraesCS7A02G064700

3,204

hydrolase activity-methyl jasmonate esterase activity-methyl salicylate esterase activity(GO:0009694), transcription regulatory region DNA binding

cellular response to auxin stimulus (GO:0071365), jasmonic acid-mediated signaling pathway (GO:0009864)

7B

TraesCS7B02G432200

3,706

potassium ion leak channel activity(GO:0016021)

potassium ion transmembrane transport-stabilization of membrane potential(GO:0016021) ,  response to light intensity (GO:0009642)

pNRT

1 A

TraesCS1A02G295400

629

water channel activity(GO:0009414), chlorophyll-binding (GO:0009579), hydrolase activity, hydrolyzing O-glycosyl compounds(GO:0005886), potassium ion leak channel activity (GO:0005774)

response to water deprivation (GO:0009414), photosynthesis, light harvesting in photosystem I- response to the light stimulus (GO:0009579), stabilization of membrane potential (GO:0005774)

TraesCS1A02G295500

261

protein self-association-unfolded protein binding(GO:0006950)

response to heat-response to hydrogen peroxide-response to reactive oxygen species-response to salt stress-response to stress (GO:0006950)

TraesCS1A02G295700

522

water channel activity(GO:0009414), potassium ion leak channel activity (GO:0065007), hydrolase activity, (GO:0005886)

response to water deprivation(GO:0009414), ), potassium ion transmembrane transport-stabilization of membrane potential(GO:0065007),

TraesCS1A02G296300

5,040

actin-dependent ATPase activity- (GO:0048765, ATP binding-protein serine/threonine kinase activity-transforming growth factor-beta receptor activity, type I(GO:0004675)

root hair cell differentiation and root hair elongation (GO:0048765) , lateral root development (GO:0048527), cellular response to growth factor stimulus (GO:0004675)

3 A

TraesCS3A02G039000

4,478

ATP binding-protein serine/threonine kinase activity (GO:0005819)

response to auxin-response to ethylene-response to gibberellin(GO:0009733), photosynthesis, light harvesting in photosystem I-response to the light stimulus (GO:0009941)

4B

TraesCS4B02G259600

3,639

ion channel binding (GO:1,903,959), sodium-independent organic anion transmembrane transporter activity (GO:0098656), protein-macromolecule adaptor activity(GO:0040008), potassium ion leak channel activity(GO:0005774), voltage-gated chloride channel activity(GO:0008308)

cellular response to starvation-positive regulation of cell growth-positive regulation of protein serine/threonine kinase activity-regulation of cell size-regulation of growth (GO:0040008) , potassium ion transmembrane transport-stabilization of membrane potential-vacuolar membrane (GO:0005774), voltage-gated chloride channel activity(GO:0008308)

TraesCS4B02G260200

2,261

potassium ion leak channel activity(GO:0016021), abscisic acid binding-protein phosphatase inhibitor activity-signaling receptor activity (GO:0050896)

potassium ion transmembrane transport-stabilization of membrane potential (GO:0016021), response to stimulus (GO:0050896)

5 A

TraesCS5A02G551700

939

hydrolase activity(GO:0005886), ATPase activity, (GO:0034605)

phenotypic switching (GO:0036166), cellular response to heat (GO:0034605) , hyperosmotic salinity response, response to auxin (GO:0009733)