Fig. 3
degS and rpoE are required for the growth S. Typhimurium growth under iron-restricted conditions. Spot dilution assay was performed with S. Typhimurium 14,028 s wild-type, ΔdegS and ΔrpoE mutants. The serial dilutions (100–10− 7 dilutions) of the cultures of wild-type, ∆ degS and ∆ rpoE, were spotted on the surface of LB agar plates containing 0 (control), 100, 200, 300, and 400 μM 2,2`-Dipyridyl (Dip). The plates were incubated at 37 °C and results were recorded after 24 hr.