Fig. 1

Workflow for SMCHD1 ChIP-seq analysis in SH-SY5Y cells. a 5-azaC treatment induces loss of DNMT1 in SH-SY5Y cells while it has no effect on SMCHD1 protein level. β-tubulin was used as an internal control for loading. b Immunoprecipitation of SMCHD1 using anti-SMCHD1 antiserum generated in guinea pig, commercial anti-SMCHD1 and guinea pig serum. Immunoprecipitated samples are blotted using anti-SMCHD1 antibody. c Schematic illustration of the workflow for analysis of the ChIP-seq data. GREAT algorithm and SeqMonk software determined distribution of SMCHD1 binding sites relative to the associated transcriptional start site (TSS). PAPST and DAVID bioinformatics identified biological processes associated with SMCHD1 occupancy, gene ontogeny, relative to the nearest gene. SeqPos (Galaxy cistrome) identified potential SMCHD1-associated binding motifs