Table 1 XX-only embryonic injection sets
Injection set | Cas9 delivery / [] | sgRNA name / [] | dsRNA name / [] | XX embryos | XX Larvae | XX Females | XX Males | XX intersexes | Cctra male-specific in XX |
|---|---|---|---|---|---|---|---|---|---|
1 | Cas9 Protein / 1,8 μg/μL | sgtraEx1 / 200 ng/μL | dsRNA-Cctra / 0.5 μg/μL | 370 | 48 | 0 | 0 | 0 | – |
2 | Cas9 Protein / 1,8 μg/μL | sgtraEx1 / 200 ng/μL | – | 400 | 60 | 6 | 6 | 0 | yes in XX males |
3 | – | – | dsRNA-Cctra / 0.5 μg/μL | 200 | 83 | 0 | 73 | 5 | – |
4 | – | sgtraEx1 / 1 μg/μL | – | 160 | 75 | 70 | 0 | 0 | no |
5 | Plasmid-dCas9 / 1 μg/μL | sgtraEx1 / 1 μg/μL | – | 290 | 37 | 7 | 0 | 3 | yes |
6 | Cas9 Protein / 1,8 μg/μL | sgtraEx1 / 200 ng/μL | – | 40 | – | – | – | – | yes |
7 | Plasmid-Cas9 / 1 μg/μL | sgtraEx1 / 1 μg/μL | – | 40 | – | – | – | – | yes |
8 | Plasmid-dCas9 / 1 μg/μL | sgtraEx1 / 1 μg/μL | – | 40 | – | – | – | – | yes |
9 | buffer | buffer | buffer | 40 | – | – | – | – | no |
10 | – | sgtraEx1 / 1 μg/μL | – | 40 | – | – | – | – | no |
11 | Cas9 Protein / 1,8 μg/μL | – | – | 40 | – | – | – | – | no |
12 | Plasmid-Cas9 / 1 μg/μL | – | – | 40 | – | – | – | – | no |
13 | Plasmid-dCas9 / 1 μg/μL | – | – | 40 | – | – | – | – | no |