Fig. 5From: CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticityStochastic allelic imbalances in cytosine methylation are exacerbated by CGGBP1 depletiona Allelic imbalances in HEK293T MeDIP are strongly different from the allelic ratios expected in the input with negligible differences between CT and KD. b and c The poor correlation between allelic imbalances in the input and CT (b) or KD (c) is evident in the heat scatters. d The stochastic allelic choices for methylation give rise to a random distribution of allelic representations in HEK293T CT and KD MeDIP (r2 = 0.361). e The allelic imbalances between GM02639 CT and KD were however highly anticorrelated as expected due to less stochasticity in these samples (r2 = 0.013). f and g Parent-of-origin specific allelic imbalance scatter plot between GM02639 CT and KD (f) shows that CGGBP1 depletion enhances a random monoallelic bias with no preference for the maternal or the paternal alleles. For all locations where the CT and KD are expected to be heterozygous ([(Pat-Mat)/(Pat+Mat)] values = 0 ± 0.5), the observed heterozygosity was very low (green shade; see text for details). Instead the unbiased monoallelic methylation with no difference between CT and KD ([(Pat-Mat)/(Pat+Mat)] values < − 0.5 or > 0.5) were observed for the majority of loci (red shades). A skewed allelic imbalance only in CT (aqua shade) or KD (pink shade). For a proportionate subset of loci, the parent-of-origin identities of the methylated alleles were reversed between CT and KD (yellow shades). g A frequency plot of the differences between percent of maternal and paternal allele contributions to the MeDIP DNA shows that CGGBP1 depletion minimizes allelic equivalence (X-axis = 0, Y-axis = − 1) and enhances exclusive maternal or paternal methylation (positive Y-axis values for X-axis = − 1 or + 1). Mat and Pat stand for Maternal and Paternal alleles respectively.Back to article page