Primer name | Nucleotide sequence (5′ to 3′) |
---|---|
For CDS cloning | |
Sox9a-F1 | ATGAATCTACTAGACCCCTACCT |
Sox9a-R1 | GCTGCTGTGCCCAATGCTG |
Amh-F1 | ATGCTCTTCCACGCAGGATTTTG |
Amh-R1 | CAAAGAGCAAAAGGAGGGTGTCA |
Cyp19ala-F1 | ATGGCAGGTGAACTTCTCCAGCC |
Cyp19ala-R1 | GAGGGCGTTTCTGGGGATGAGC |
For 3′ RACE | |
Sox9a-3′-F | CAACTCCTATTACACCCACGCT |
Amh − 3′-F | CAACAACCACGCCATCCTC |
Cyp19ala − 3′-F | TGCTCAAACAGAATCCAGAC |
 3′-adaptor Primer | CTGATCTAGAGGTACCGGATCC |
For RT-qPCR | |
β-actin-F | CATCTACGAGGGTTACGCCC |
β-actin-R | AATTTCCCTCTCGGCTGTGG |
Sox9a-F2 | TCAATACCCGCACCTCCACAACGCC |
Sox9a-R2 | TGAAGATGGCGTTCGGGGAGATGTG |
Amh-F2 | GGAATTCACCAGTCCTGATAGC |
Amh-R2 | CTGCAGAAGTTCTTGAGTGACG |
Cyp19ala-F2 | TTGACACCTGGCAGACGGTA |
Cyp19ala-R2 | CTGCGATTATCATCTCCAACAC |