Schematic of the mutation identification in lbab mice. A: A genetic map of the lbab locus showing the relative locations of microsatellite markers and the total number of candidate transcripts within the lbab locus. B: PCR product analyses using the SpectruMedix system. The PCR products from different species of mice were pair mixed to check the possible sequence difference between normal B6 and PL/J mice (+/+), normal and homozygous lbab mice (mixture of +/+ and lbab/lbab), intrahomozygous lbab mice (lbab/lbab), and intraheterozygous mice (+/lbab). C: The PCR amplification products of exon 2 of the Nppc gene from 11 strains of mice (C57BL/6J, SJL/J, CAST/Ei/J, C3H/HeJ, PL/J, BALB/cJ, RF/J, KK/H1J, A/J, DBA/1J, and BTBR/J). D: PCR products from each of those strains were mixed separately with that of lbab/lbab mice. Each mixture showed multiple bands of signal, indicating the difference in their DNA sequences. The X-axis represents relative size of the PCR products. The Y-axis represents relative strength of signal or the amount of the PCR products.