Fig. 1From: The choice of negative control antisense oligonucleotides dramatically impacts downstream analysis depending on the cellular backgroundOverview of the experimental procedure. (a) Schematic representation of the experimental workflow. LECs and BECs were subjected to ASO-mediated knockdown (ASOKD) followed by Cap Analysis of Gene Expression (CAGE-Seq). Only samples with a knockdown efficiency higher than 50% in both replicates were subjected to CAGE-Seq. (b) Bioinformatic pipeline highlighting the quality control steps prior to the transcriptome profiling after lncRNA candidate knockdownsBack to article page