Fig. 5
From: The transcription factor reservoir and chromatin landscape in activated plasmacytoid dendritic cells
TFs show CpG-dependent expression and chromatin accessibility. A Number of differentially accessible peaks of genomic regions associated with TF genes detected using DESeq2 comparing naïve to 2 h CpG stimulated pDCs, |FC| > 2 and p < 0.05. B Heatmap of normalized ATAC-Seq peak intensities (log2FC relative to the mean for each peak) limited to 540 peaks from (A) that are condition-dependent with |FC| > 2 and p < 0.05 for at least one pairwise comparison of interest. C The bar graph depicts normalized expression values obtained from RNA-Seq and statistics calculated with edgeR. D, E Top panel presents screen shots from the ECR (evolutionary conserved regions) Browser web site of the respective indicated gene. Exonic regions are shown in blue, intronic regions in pink, UTRs in yellow, and CNS in red. Bottom panels present ATAC-Seq peaks in naïve and CpG stimulated (2 h) pDCs for the indicated genes visualized with IGV. The AP-1 motif within the promoter sequence of the Tcf4 gene is highlighted in (E)