From: Accumulation of genetic variants associated with immunity in the selective breeding of broilers
 | Sequencing Statistics | Summary of SNPs Identified By Feature and Impact | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sequencing Method | Total # of reads | Aligned Reads | Unmapped | Breadth of coverage | Average read depth | Synonymous | Non-synonymous | Intergenic | Intronic | UTR5 | UTR3 | Frameshift indel | Non-frameshift indel | Stop gain | Stop loss | |
1957 | NextSeq | 954,402,120 | 771,590,591 | 178,041,952 | 90% | 89 | 63,573 | 28,697 | 2,733,442 | 3,917,443 | 50,831 | 139,826 | 2993 | 973 | 203 | 38 |
1978 | NextSeq | 956,913,758 | 813,956,611 | 124,470,402 | 90% | 91 | 70,413 | 31,930 | 2,953,408 | 4,317,698 | 57,084 | 151,967 | 3145 | 1013 | 225 | 50 |
Ross 308 | NextSeq | 753,170,974 | 688,175,109 | 20,392,933 | 90% | 83 | 70,208 | 31,791 | 2,933,982 | 4,283,304 | 56,713 | 152,151 | 3030 | 1006 | 245 | 47 |
Silkie | Illumina GAII | 250,344,910 | 183,908,065 | 4,059,840 | 91% | 16 | 18,035 | 5120 | 5,769,436 | 1,141,875 | 2571 | 25,457 | 191 | 111 | 11 | 2 |
L2 | Illumina GAII | 319,254,874 | 226,413,441 | 4,765,903 | 93% | 19 | 18,131 | 5206 | 5,804,968 | 1,141,600 | 2829 | 25,680 | 226 | 121 | 14 | 2 |
CB1 | SOLiD | 302,271,430 | 191,347,790 | 99,593,871 | 90% | 8 | 8444 | 2085 | 2,009,820 | 404,234 | 646 | 9286 | 136 | 121 | 6 | 1 |
CB2 | SOLiD | 225,049,549 | 138,854,319 | 78,806,791 | 89% | 3 | 4945 | 1171 | 1,270,404 | 262,783 | 329 | 5916 | 73 | 63 | 3 | 0 |
High | SOLiD | 285,510,087 | 186,711,973 | 89,131,046 | 86% | 4 | 3874 | 1005 | 1,058,205 | 218,944 | 245 | 4974 | 67 | 64 | 1 | 0 |
Low | SOLiD | 311,562,452 | 201,484,147 | 98,719,567 | 88% | 4 | 2419 | 630 | 706,923 | 146,740 | 173 | 3255 | 22 | 18 | 1 | 0 |
Obese | SOLiD | 214,645,321 | 127,085,837 | 80,434,294 | 89% | 6 | 5203 | 1313 | 1,309,898 | 267,762 | 395 | 5958 | 80 | 71 | 3 | 0 |
RIR | SOLiD | 182,411,411 | 111,322,051 | 64,677,509 | 89% | 5 | 3996 | 989 | 972,200 | 196,322 | 257 | 4490 | 46 | 40 | 1 | 2 |
WLA | SOLiD | 311,017,596 | 176,885,293 | 101,265,637 | 91% | 7 | 7334 | 1959 | 1,972,780 | 406,528 | 650 | 9128 | 143 | 130 | 4 | 1 |
WLB | SOLiD | 179,401,459 | 132,406,702 | 39,517,064 | 88% | 5 | 5640 | 1465 | 1,562,666 | 319,525 | 396 | 7104 | 93 | 86 | 1 | 0 |
RJF | SOLiD | 176,071,566 | 112,046,668 | 57,045,807 | 88% | 5 | 309 | 80 | 66,657 | 12,527 | 14 | 324 | 18 | 15 | 0 | 0 |
RJFswe | SOLiD | 355,473,025 | 254,348,419 | 131,460,569 | 91% | 10 | 10,677 | 2688 | 2,544,261 | 516,481 | 903 | 11,729 | 178 | 159 | 7 | 1 |