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Table 1 Sequencing and genomic variant statistics for breeds used in this study

From: Accumulation of genetic variants associated with immunity in the selective breeding of broilers

 

Sequencing Statistics

Summary of SNPs Identified By Feature and Impact

Sequencing Method

Total # of reads

Aligned Reads

Unmapped

Breadth of coverage

Average read depth

Synonymous

Non-synonymous

Intergenic

Intronic

UTR5

UTR3

Frameshift indel

Non-frameshift indel

Stop gain

Stop loss

1957

NextSeq

954,402,120

771,590,591

178,041,952

90%

89

63,573

28,697

2,733,442

3,917,443

50,831

139,826

2993

973

203

38

1978

NextSeq

956,913,758

813,956,611

124,470,402

90%

91

70,413

31,930

2,953,408

4,317,698

57,084

151,967

3145

1013

225

50

Ross 308

NextSeq

753,170,974

688,175,109

20,392,933

90%

83

70,208

31,791

2,933,982

4,283,304

56,713

152,151

3030

1006

245

47

Silkie

Illumina GAII

250,344,910

183,908,065

4,059,840

91%

16

18,035

5120

5,769,436

1,141,875

2571

25,457

191

111

11

2

L2

Illumina GAII

319,254,874

226,413,441

4,765,903

93%

19

18,131

5206

5,804,968

1,141,600

2829

25,680

226

121

14

2

CB1

SOLiD

302,271,430

191,347,790

99,593,871

90%

8

8444

2085

2,009,820

404,234

646

9286

136

121

6

1

CB2

SOLiD

225,049,549

138,854,319

78,806,791

89%

3

4945

1171

1,270,404

262,783

329

5916

73

63

3

0

High

SOLiD

285,510,087

186,711,973

89,131,046

86%

4

3874

1005

1,058,205

218,944

245

4974

67

64

1

0

Low

SOLiD

311,562,452

201,484,147

98,719,567

88%

4

2419

630

706,923

146,740

173

3255

22

18

1

0

Obese

SOLiD

214,645,321

127,085,837

80,434,294

89%

6

5203

1313

1,309,898

267,762

395

5958

80

71

3

0

RIR

SOLiD

182,411,411

111,322,051

64,677,509

89%

5

3996

989

972,200

196,322

257

4490

46

40

1

2

WLA

SOLiD

311,017,596

176,885,293

101,265,637

91%

7

7334

1959

1,972,780

406,528

650

9128

143

130

4

1

WLB

SOLiD

179,401,459

132,406,702

39,517,064

88%

5

5640

1465

1,562,666

319,525

396

7104

93

86

1

0

RJF

SOLiD

176,071,566

112,046,668

57,045,807

88%

5

309

80

66,657

12,527

14

324

18

15

0

0

RJFswe

SOLiD

355,473,025

254,348,419

131,460,569

91%

10

10,677

2688

2,544,261

516,481

903

11,729

178

159

7

1