Building a transgenic sexing strain for genetic control of the Australian sheep blow fly Lucilia cuprina using two lethal effectors

Table 1 Rearing efficiency and female lethality of L. cuprina TSS

TSS	Condition	Tet^a	#Pupae	#Male	#Female	#Adult	AER^b	%Male
FL3#2 ^c	Heterozygous	–	2098	644	735	1379	65.7	46.7%
	Heterozygous	+	2468	1068	1048	2116	85.1	50.5%
	Homozygous	–	2387	1029	1	1030	43.1	99.9%
	Homozygous	+	1647	672	701	1373	83.3	49.0%
FL3#2 ^d	Homozygous	–	1736	881	2	883	50.9	99.8%
FL3#2 ^d	Homozygous	+	1562	629	685	1314	84.1	47.9%
DH6 ^d (FL3#2; EF1#12)	Heterozygous	–	1717	1383	0	1383	80.5	100%
	Homozygous	–	427	270	0	270	63.2	100%
		++/−	1421	885	0	885	62.3	100%
		+	1611	719	669	1388	86.2	51.8%
DH1 ^e (DR2#6; EF1#12)	Heterozygous	–	1091	916	0	916	84	100%
	Homozygous	+/−	1054	958	0	958	90	100%
	Homozygous	+	2719	1284	1234	2518	92.6	51.0%
DH2 ^e (DR2#6; EF3E)	Heterozygous	–	1252	1099	0	1099	87.8	100%
	Homozygous	+/−	694	594	0	594	85.6	100%
	Homozygous	+	1724	784	738	1522	88.3	51.5%
DH3 ^f(DR3#2; EF3E)	Heterozygous	–	1082	771	0	771	71.3	100%
	Homozygous	+/−	1158	981	0	981	84.7	100%
	Homozygous	+	1949	838	810	1648	84.6	50.8%
DH4 ^f(DR3#4; EF1#12)	Heterozygous	–	2398	1690	30	1720	71.7	98.3%
	Homozygous	+/−	953	801	0	801	84.1	100%
	Homozygous	+	2284	1039	1008	2047	89.6	50.8%
DH5 ^f (DR5#4; EF1#12)	Heterozygous	–	1271	1132	0	1132	89.1	100%
	Homozygous	–	660	561	0	561	85	100%
	Homozygous	+	2162	981	881	1862	86.1	52.7%

^a “-” stands for no tetracycline in the diet, and “+” stands for plus tetracycline in the diet, “+/−” indicates parents fed a low dose of tetracycline (1 or 3 μg/mL for the first 2 days), “++/−” indicates a high dose of tetracycline (100 μg/mL) was supplied to the parental adults for the first 8 days but not their progeny that were counted
^bAER stands for adult emergence ratio
^cData from [20]. Eggs were collected up to two times from 10 to 20 pairs of adults
^dData from this study, three replicates of 8-pairs per cage
^eData from [12]. For DR2 the bottleneck (bnk) cellularization gene promoter from L. sericata was used to drive expression of tTA. EF3 contains the wild type version of Lshid whereas EF1 has a phosphomutated version called Lshid^Ala2
^fData from [31]. The spitting image (spt) gene promoter from L. sericata and the actin5C gene promoter from L. cuprina was used to drive expression of tTA for DR3 and DR5, respectively

ISSN: 2730-6844