Fig. 3

Female-specific lethality of FL3#2 (a) and DH6 (b) under different tetracycline feeding regimens. Containers were set with eight pairs of adults and the number of third instar (L3), pupae and adult male and female offspring were counted. +W: parental generation fed water with 100 μg/mL tetracycline from day 1 (D1) to D8; −W: parental generation fed water without tetracycline from D1 to D8; +M: ground meat (larval diet) with 100 μg/g tetracycline; −M: meat without tetracycline. Each experiment was performed three times. Mean ± standard deviation are shown