Table 3 Description, annotation and qPCR characteristics of selected transcripts
ID/ gene namea | GO term assigned | Accession no.b | Primer sequence 5′-3′ | Product size (bp) | qPCR efficiency |
|---|---|---|---|---|---|
Development | |||||
Trinity DN12595_c0_g1_i2/ serendipity alpha | 0016020/ 0007349/ 0005737 | JQ599256 | Fw: TGAGAGTTTGCGCACAGTGA Rv: CACTGCTGCACCTGAGTCAT | 154 | 1.882 |
Trinity DN15480_c0_g1_i3/ transformer-2 | – | KY204955 | Fw: TGGAAATCGATGATCGCCGT Rv: GACGACGGGAGCGATAATCA | 144 | 1.957 |
Trinity DN10186_c0_g1_i4/ heat shock protein 27 | 0005875/ 0008340/ 0042026/ 0042595 | XM011197636 | Fw: TTTCGGCTTTGGCTTGCATC Rv: TGCACACTTGGAAGCCATCT | 200 | 1.929 |
Courtship behavior/communication | |||||
Trinity DN11516_c0_g1_i3/ takeout-like | – | JQ048622 | Fw: TTTACGGCGTTGGTAGTGCC Rv: CCGATCAACTCAGCTTTGGG | 106 | 1.924 |
Trinity DN11027_c0_g1_i1/ odorant-binding protein 50a1 | – | KU317977 | Fw: GCTGCAGTCGTTGTCCATTG Rv: GTGTCGACATGCAACTCAGC | 85 | 1.934 |
Physiological pathways | |||||
Trinity DN15916_c0_g4_i3/ androgen-induced gene 1 protein | 0012505/ 0016021 | XM029042282 | Fw: GCGTGTTGGATGCAGTGTTT Rv: CGTTTGGGATAGGCACGGAA | 109 | 1.905 |
Trinity DN12527_c0_g1_i1/ maltase 2-like | – | XM011180327 | Fw: CCAGCCCGAATACGGTACAA Rv: CGGTCGACGGACTTCTGAAA | 138 | 1.907 |
Reference genes | |||||
Trinity DN6544_c0_g1_i2/ ribosomal protein L18 | 0000022/ 0003735/ 0022625/ 0006412 | NM139834 | Fw: GAAAGGCTCCTGGTGTTCCA Rv: TTTGTAACCGCAGCTTGACC | 104 | 1.963 |
Trinity DN12142_c0_g1_i2/ elongation factor - 1α | 0008340/ 0003746/ 0006414/ 0005525/ 0006184/ 0005829/ 0005853 | GU339154 | Fw: GCACCACGAAGCTTTAGCAG Rv: ACTGGAGTGTAACCGTTGGC | 198 | 1.959 |