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Table 1 (a) results of inheritance mode experiments of mutants, (b) linkage analysis of red body (rb), white eye (we) and black pupae (bp) mutants and (c) GSS backcrossing to we and rb alleles in Anastrepha fraterculus sp. 1

From: Development and characterization of a pupal-colour based genetic sexing strain of Anastrepha fraterculus sp. 1 (Diptera: Tephritidae)

Inheritance crosses

F1 phenotype

F2 phenotypes

Total

X2 (3:1)

Wild type

mutant

rb

WT

all population WT

302

297

91

88

778

1.65

WT

rb

240

188

76

87

591

2.10

we

WT

259

245

69

72

645

3.39

WT

we

119

108

33

31

291

1.40

bp

WT

528

511

190

194

1423

0.16

WT

bp

446

439

155

148

1188

2.99

Linkage crosses

F1 phenotype

F2 phenotypes

Total

X2 (9:3:3:1)

WT

mutant

mutant

double mutant

we

bp

all population WT

997

3201

3612

75

1753

14.43

rb

bp

1002

3443

3632

82

1791

10.43

rb

we

559

1923

1771

61

989

0.63

Backcrossing

WT

rb

we

rb we

Total

♂ F1

GSS-89

rb we

250

241

181

183

231

211

216

187

1700

  1. Hypothesis 3:1, X20.05, 1 = 3.841
  2. Hypothesis 9:3:3:1, X20.05, df = 3 = 7.82
  3. WT Wild type; mutant1 = we; mutant2 = bp; mutant3 = rb