Fig. 2From: Characterization of TMC-1 in C. elegans sodium chemotaxis and sodium conditioned aversionThe effects of sodium conditioning on chemotaxis. Each individual chemotaxis assay contained at least 100 nematodes, data reported here as average of independent assays (n). Each data point represents a minimum of three assays. Nematodes were conditioned for 20 min in either CTX buffer or CTX buffer containing 50 mM NaCl. Chemotaxis index (C.I) has a range of − 1 to 1, where positives values indicate attraction to tastant, negative values indicate aversion, and values close to 0 indicate no preference. Error bars represent SEM. (a) Sodium conditioning disrupts wild-type nematodes’ NaCl and LiCl attraction pathway. Sodium conditioned N2 nematodes behaved significantly different from their naïve counterpart toward 50 mM NaCl (n: naïve = 6, conditioned = 5) and 50 mM LiCl (n: naïve = 4, conditioned = 4). There was no statistically significant difference in their behavior toward 10 mM lysine (n: naïve = 5, conditioned = 5). For this and panel B, asterisks identify significant conditioning effects as indicated by unpaired Student’s t-test (p < 0.05). (b).Tmc-1 mutation disrupts nematodes’ innate attraction and induced aversion to 50 mM NaCl. (n: CGnaïve = 5, CGconditioned = 5, RBnaive = 4, RBcondtioned = 4)Back to article page